3D Prime Price Reduction: As a result of increasing demand and improved economies of scale, we are reducing the price of CnT-02-3DP5 medium effective 1st April 2012.  Check out the updated price lists on the appropriate ordering page for more information.

3D Starter Kits

Containing everything you need to establish 3D epidermal cultures routinely in your lab, our 3D starter kits are unique in the market. Click on the links for the product data sheets.

Each kit comes with a complete protocol, explaining step-by-step how to establish the models. In addition, a training video is also available, which demonstrates the written protocol visually (see our resources page).

Media for 3D Epidermal Cell Culture

Improving still further our originial medium for 3D culture of epidermal keratinocytes, CELLnTEC has now released a new formulation, known as 3D Prime. Click on the links for the product data sheets.

CELLnTEC 3D Cell Culture

Three dimensional (3D) epidermal keratinocyte cultures remain the gold-standard for highly accurate in vitro modeling of the epidermis. With a fully stratified structure extending from proliferative basal progenitors through fully differentiated cornified cells, these models are able to reproduce most of the intricate functions of the in vivo epidermis. 

However the establishment of such a stratified model places very specific and quite divergent demands on the cell culture medium. In particular, the medium must support cells to reach terminal differentiation as they stratify and form the suprabasal layers of the model, whilst in parallel retaining a population of proliferative cells in the basal layer.

CELLnTEC is the first company to provide a specialized culture medium specifically tuned to the unique demands of a 3D keratinocyte culture. Known as 3D Prime, this fully defined medium was developed using our unique signaling based approach. It enables the routine and reliable establishment of a fully stratified epithelium in just 14 to 18 days air-lifted culture.

The 3D Prime medium is also available as part of a kit containing  all the components necessary for the establishment of 3D epidermal models in your own lab.
Also available: a full training video demonstrating our recommended written protocol for the establishment of 3D epidermal cell cultures.

Markers Expression and Lipid Profile

For a in vivo / in vitro comparison of proliferation and differentiation markers as well as a comparison of how the lipid profiles compare, please click here.

Free Samples

CELLnTEC is offering free samples to new customers who are interested to test the performance of our precision culture media.

This sample comes as 100 mL supplemented CnT-02-3D Prime medium, which is ready to use. There is no need for additional components. It also includes 6 inserts for lifted culture and 1 mL each of our Stain Solution I and II for confirming confluency.

Important Note: When testing your free sample, it is very important that the keratinocytes used are proliferating well in one of our PCT media (i.e. CnT-07 or CnT-57). If you are not yet using our keratinocyte media for your primary keratinocyte cell culture, we strongly recommend to order a free sample of these media as well. Follow this link to read more about our epidermal culture media, with more info to KMSPSAMPLE, our Keratinocyte Media Sample Pack, at the bottom of that page.