Developed specifically for the unique demands of 3D culture, our new 3D Prime culture medium now allows you to establish accurate 3D epidermal models routinely and cost effectively in your own lab.

The medium is available separaptely, or alternatively as part of a convenient 3D starter kit that containing everything needed to establish your own 3D epidermis, in your lab, to your schedule. Free samples are also available.

3D Starter Kits

Containing everything you need to establish 3D epidermal cultures routinely in your lab, our 3D starter kits are unique in the market. Click on the links for the product data sheets.

Each kit comes with a complete protocol, explaining step-by-step how to establish the models.  In addition, a training video is also available, which demonstrates the written protocol visually (see our resources page).

Media For 3D Epidermal Cell Culture

Improving still further our originial medium for 3D culture of epidermal keratinocytes, CELLnTEC has now released a new formulation, known as 3D Prime. Click on the links for the product data sheets.

3D epidermal culture established using the 3D starter kit and 3D Prime culture medium

Developed using our established signaling-based approach, this completely defined medium enables more rapid establishment of a fully stratified epidermis. Using this medium, models can now be ready for experimentation in just 14 to 18 days.

Like all our media, CnT-02-3DP is supplied as a kit with all the required supplements, and needs no further supplementation.

In addition, these media are also available in 3D starter kits, which include all the reagents necessary for you to create 3D models routinely in your own lab according to a full written protocol.

For a in vivo / in vitro comparison of two proliferation and differentiation markers as well as a comparison of how the lipid profiles compare, please click here.

Free Samples

CELLnTEC is offering free samples to new customers who are interested to test the performance of our precision culture media.

This sample comes as 100 mL supplemented CnT-02-3D Prime medium, which is ready to use. There is no need for additional components. It also includes 6 inserts for lifted culture and 1 mL each of our Stain Solution I and II for confirming confluency.

Important Note: When testing your free sample, it is very important that the keratinocytes used are proliferating well in one of our PCT media (i.e. CnT-07 or CnT-57). If you are not yet using our keratinocyte media for your primary keratinocyte cell culture, we strongly recommend to order a free sample of these media as well. Follow this link to read more about our epidermal culture media, with more info to KMSPSAMPLE, our Keratinocyte Media Sample Pack, at the bottom of that page.