CELLnTEC has the following specialized mammary epithelium media available for your cell culture.

Human primary mammary epithelium cells grown in CnT-54, after 5 population doublings (cells grown according to our recommended protocols and seeding densities)

Isolation & Proliferation Media

CnT-27 - Defined, PCT medium where a completely defined environment and maximum mammary epithelial cell purity is required. The PCT formulation of CnT-27 provides excellent results through maximum retention of proliferative mammary epithelial progenitor cells. 

CnT-54 - Low BPE, PCT medium recommended where the absolute best isolation efficiency, colony formation efficiency and longevity is required, and a low BPE concentration (5 to 8 x lower than competitors) is compatible with the experimental setup.

Differentiation Media

CnT-22 - Defined medium using a highly optimized basal formulation and supplements, but does not contain PCT factors. Intended for use in situations where mammary cells are induced to differentiate.

As with all differentiation work, it is important that cells reach confluency before calcium levels are increased. Furthermore, and next to the addition of calcium, cells should also be switched to CnT-02, a non-PCT medium. For our recommended differentiation protocol, see our resources section.

Applications from the Literature

Isolation and Cultivation of Human Mammary Epithelial Cells: For a sample publication of the use of CELLnTEC's Mammary Media, please see Kocanova et al., 2010, PLOS Genetics.

Generation of Mammospheres: To see how our media was successfully applied to grow mammospheres with a human breast carcinoma cell line, please see Prud'homme et al., 2010, PLOS One.

Available Sample of Mammary Epithelium Culture Media

CELLnTEC is offering free samples to new customers who are interested to test the performance of our precision culture media.

When testing your free sample, there are a number of important considerations. Please see our resources section for Sample Testing Recommendations.

Order your Free Sample Today: Please contact your local distributor for a free sample. Please see our ordering section for the CELLnTEC product distributor in your area.