Cultivation Protocols

Recommended Seeding Densities (PDF)

General Cultivation Protocol for Primary and Long-Term Cells (PDF)

Isolation Protocols

Recommended Seeding Densities (PDF)

Epidermal Keratinocyte Isolation, Human (PDF)

Epidermal Keratinocyte Isolation, Mouse (PDF)

Epidermal Keratinocyte Isolation, Dog (PDF)

Airway (Small) Epithelium Cell Isolation, Human (PDF)

Airway (Large) Epithelium Cell Isolation, Human (PDF)

Bladder Epithelium Cell Isolation, Human (PDF)

Bladder Epithelium Cell Isolation, Rat (PDF)

Corneal Epithelium Cell Isolation (PDF)

Fibroblast Isolation, Human (PDF)

Mammary Epithelium Cell Isolation (PDF)

Melanocyte Isolation (PDF)

Oral Epithelium Cell Isolation (PDF)

Prostate Epithelium Cell Isolation (PDF)

3D Spheroid Protocols

Establishment of 3D fibroblast cultures (PDF)

2D Differentiation Protocols

2D Differentiation of Epidermal Keratinocytes in CnT-07 / CnT-57 (PDF)

2D Differentiation of Other Epithelial Cells grown in a PCT medium (PDF)

3D Differentiation Protocols

For the 3D Epidermal Cell Culture Training Video, please click here.

3D Epidermal Cell Cultures: Starter Kit with CELLnTEC HPEK Keratinocytes (PDF)

3D Epidermal Cell Cultures: Starter Kit without CELLnTEC Keratinocytes (PDF)

3D Epidermal Cell Cultures: Mouse Keratinocytes (PDF)

Routine Histology and Staining of 3D Cell Cultures (PDF)

OCT Embedding of 3D Cultures for Cryo-Sectioning (PDF)

Other Useful Protocols

Lipid Extraction (PDF)

Keratinocyte Transfection Using PEI (PDF)

General Staining Protocol (PDF)

CELLnTEC Media Composition

For more info to CELLnTEC's Media Composition, click here.