PCT Bladder Epithelium Medium, Low BPE
CnT-58 is a progenitor cell targeted liquid culture medium kit including both basal medium and supplements. CnT-58 contains a low Bovine Pituitary Extract (BPE) concentration of 6 μg / mL. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
CnT-58 is a 500 mL kit consisting of:
CnT-BM.58: 500 mL CnT Basal Medium 1 (liquid)
CnT-58.S: containing four supplements (Supplements A, B, C and BPE; delivered frozen; to be added to 500 mL basal medium prior to use)
Developed for maximum efficiency in bladder epithelium cell isolation and growth. With the additional boost of BPE, this media is also recommended for use with sub-optimal conditions such as low seeding densities or low tissue quality.
For 2D differentiation experiments we recommend to use CnT-21 Bladder Epithelium Medium.
Formulated for human and mouse epithelium cells. May be used for other species as well (not yet tested).
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.
CnT-BM.1 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.
CnT-58.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag.
The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 8 weeks. Please do not freeze.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Media functionality and cell morphology tested by cell cultivation over 2 passages in supplemented medium. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
CnT-BM.1 basal medium is shipped at ambient temperature.
CnT-58.S supplements are shipped on dry ice.
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
For research use only. Not for use in therapy or diagnostics.