RBLAK-WIS
Printable Datasheet/Page
Product Title:
Bladder Epithelium Progenitors
Rat bladder epithelium progenitor cells, cryo preserved at passage 25. Long-Term cells supplied with one 500 mL kit of medium (basal medium with frozen supplements).
Species:
Rat, Wistar
Pack Size:
RBLAK-WIS is a kit consisting of:
- 1 x RBLAK-WIS.C: > 5 x 105 viable cells per vial (1mL)
- 1 x CnT-16: 500 mL kit of CnT-16 medium
Culture Medium:
Included medium in the kit:
CnT-16, PCT Bladder Epithelium Medium, Defined
Alternatively cells can also be cultivated in the medium listed below:
CnT-36, Bladder Epithelium Differentiation Medium, Defined
Cultivation:
For cultivation instructions, please see general cultivation protocol, on our recources section.
Passaging:
Recommended seeding density after passaging: 2 x 104 cells / cm2.
For passaging instructions, please see general cultivation protocol, on our resources section.
Av. Time to Confluence:
5 to 7 days (depending on temperature, seeding density and protocol)
Longevity:
Guaranteed to provide an additional 6 months of continuous growth when used with the recommended medium.
Storage / Shelf Life:
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Medium storage: refer to medium label and data sheet.
Thawing:
Recommended seeding density after thawing: 1 to 3 x 104 cells / cm2.
For thawing instructions, please see general cultivation protocol, on our resources section.
Freezing:
Recommended feezing density: 1 x 106 cells / mL.
For freezing instructions, please see general cultivation protocol, on our resources section.
Quality Control:
Free of bacteria, fungi and mycoplasma contamination.
Shipping Conditions:
Cells and supplements are shipped on dry ice.
Basal medium is shipped at ambient temperature.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Mon, 2010/06/07
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For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.