HBEP.15
Printable Datasheet/Page
Product Title:
Bladder Epithelium Progenitors, Single Donor
Normal human bladder progenitor cells, cryo preserved at P2. Primary cells supplied with one 500 mL kit of medium (basal medium with frozen supplements).
Warning:
This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species:
Human, single donor, bladder
Pack Size:
HBEP.15 is a kit consisting of:
- 3 x HBEP.05.C: 3 vials > 2.2 x 104 cell clumps per vial, equates > 5 x 105 viable cells (1 mL)
- 1 x CnT-58: 500 mL kit of CnT-58 medium
Culture Medium:
Included medium in the kit:
CnT-58, PCT Bladder Epithelium Medium, Low BPE
Alternatively cells can also be cultivated in the media listed below:
- CnT-18, PCT Bladder Epithelium Medium, Defined
- CnT-21, Bladder Epithelium 2D Differentiation Medium, Defined
Cultivation:
For cultivation instructions, please see general cultivation protocol on our resources section.
Passaging:
Recommended seeding density after passaging: CnT-58: 4 x 103 cells / cm2; CnT-18: 4 x 103 cells / cm2 .
For passaging instructions, please see special bladder cultivation protocol on our resources section.
Av. Time to Confluence:
10 to 14 days after thawing and 7 to 10 days subsequently (depending on temperature, seeding density and protocol)
Req. Reagents:
Accutase for detachment of single cells, CnT-Accutase-100
Longevity:
Guaranteed to provide 15 population doublings when grown in a PCT medium.
Storage / Shelf Life:
Immediately upon arrival, transfer the cryo vial to the liquid nitrogen container until ready to use.
Medium storage: refer to medium label and data sheet.
Thawing:
Recommended seeding density after thawing: CnT-58: 100 cell clumps / cm2; CnT-18: 300 cell clumps / cm2 .
For thawing instructions, please see general cultivation protocol on our resources section.
Freezing:
Recommended freezing density: 1 x 106 cells / mL.
For freezing instructions, please see general cultivation protocol on our resources section.
Quality Control:
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping Conditions:
Cells and supplements are shipped on dry ice.
Basal medium is shipped at ambient temperature.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Thu, 2011/06/16
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Bladder cells are sensitive to trypsin and trypsin like enzymes. The use of these enzymes impairs the cell attachment and cell growth, therefore CELLnTEC recommends the use of Accutase for detachement.
For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics. For isolation, however, we recommend the use of antibiotics / antimycotics up to passage 2.