CELLnTEC has the following specialized corneal epithelium media available for your cell culture.

Isolation & Proliferation Media

CnT-20 - Defined, PCT medium. Where a completely defined environment and maximum corneal epithelium cell purity is required, the PCT formulation of CnT-20 provides excellent results through maximum retention of corneal epithelial progenitor cells.

CnT-50 - Low BPE, PCT medium recommended where absolute best attachement and isolation efficiency, colony formation and longevity is required, and a low BPE concentration (5 to 8 x lower than competitors) is compatible with the experimental setup.

Differentiation Media

CnT-30 - Defined medium using a highly optimized basal formulation and supplements, but does not contain PCT factors. Intended for use in situations where corneal cells are induced to differentiate, to avoid the differentiation delay which may occur with PCT media.

Comparisons

A comparison of culture media for isolation and culture of limbal epithelial cells: A study done at Baylor College in Houston, Texas (presented at ARVO 2007) showed that this medium provides better isolation, growth, and stem cell marker expression than alternative media from Invitrogen, Cambrex, and Cascade Biologics (click here for the full poster).

Applications From The Literature

Human primary corneal epithelium cells grown and used for 3D cell sheet formation: A comparison of growth and morphology in CnT-50 versus CnT-20 and the use of CELLnTEC media for 3D cell sheet formation can be found in a publication by Kobayashi et al., 2009, Molecular Vision.

Limbal epithelial graft generation and transplantation: A recent publication has also described the use of CnT-20 in a clinical setting, for transplantation of limbal cells (see Zacharia et al., 2010, Tissue Engineering).

In vitro corneal epithelial cell wound healing: For an example of an in vitro corneal epithelial cell wound healing assay, see the publication of Yoshioka et al., 2010, Investigative Ophthalmology and Visual Science.

De-differentiation and support of progenitor cells: In an poster abstract from Chuang et al., 2008, presented at the ARVO meeting, the results of a study about de-differentiation of corneal epithelial cells are discussed.

To search our full list of publications for your own keywords, please visit our Publications Page.

Available Sample of Corneal Epithelium Culture Media

CELLnTEC is offering free samples to new customers who are interested to test the performance of our precision culture media.

When testing your free sample, there are a number of important considerations. Please see our resources section for Media Testing Recommendations.

Order your Free Sample Today: Please contact your local distributor for a free sample. Please see our ordering section for the CELLnTEC product distributor in your area.