Using precision approaches to media and model development, CELLnTEC has created a wide range of products for the culture of primary epidermal keratinocytes. Media are tailored for the culture of human and animal cells, in both 2D and 3D culture.

For more detailed information, please see the following links:

Comparisons and Applications

Please visit the Culture Media page for comparisons of CELLnTEC media with other formulations, as well as exciting new applications from the literature, for example in the fields of:

• Epithelial stem cell isolation, and co-culture with stromal cells
• A comparison of 4 different culture media for isolation and culture of basal keratinocytes
• Co-culture of keratinocytes and melanocytes
• Stem and transient amplifying cell biology in aged epidermis
• Use of primary keratinocytes to generate iPS cells
• 3D culture of mouse keratinocytes

For a more complete list of publications in the skin field, please visit our searchable Publications page.

Human Epidermal Keratinocytes (HPEKp) 4 days after thawing in CnT-57 (cells grown according to our recommended protocols and seeding densities)

Human Epidermal Keratinocytes (HPEKp) in CnT-57 after 20 population doublings (cells grown according to our recommended protocols and seeding densities)