3D-HPEK-25
Printable Datasheet/Page
Product Title:
3D Keratinocyte Starter Kit including Human Primary Keratinocytes
3D keratinocyte starter Kit was developed for the establishment of 3 dimensional keratinocyte cultures on a porous membrane. Primary cells are cryo preserved at P2 and supplied with one 500 mL kit of CnT-57 medium (basal medium with frozen supplements), two 100 mL kits of CnT-02-3DP1 (basal medium with frozen supplements), stain solution and Millicell cell culture inserts.
Warning:
The cells are of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species:
Keratinocytes: Human, pooled (3 or more donors), neonatal foreskin
Kit is developed for human keratinocytes but may be used for other species as well.
Pack Size:
3D-HPEK-25 is a kit consisting of:
- 1 x HPEKp.05.C: Human Epidermal Keratinocyte Progenitors > 5 x 105 viable cells per vial (1 mL)
- 1 x CnT-57: 500 mL kit of CnT-57 PCT Epidermal Keratinocyte Medium, Low BPE
- 2 x CnT-02-3DP1: two 100 mL kits of CnT-02-3DP1 Epidermal Keratinocyte 3D Prime Medium, Defined
- 25 x Millipore Millicell-PCF inserts: standing cell culture inserts, polycarbonate membrane, 12 mm diameter, 0.4 µm pore size, sterile and individually blistered
- 1 x CnT-ST: stain solution with 3 mL stain solution I and 3 mL stain solution II
- 1 x 3D-HPEK-25 Data sheet
- 1 x 3D-HPEK-25 Protocol
Culture Medium:
Included medium in the kit:
CnT-57, PCT Epidermal Keratinocyte Medium, Low BPE
CnT-02-3DP1, Epidermal Keratinocyte 3D Prime Medium, Defined, 100 ml
Alternatively cells can also be cultivated in the media listed below:
- CnT-07, PCT Epidermal Keratinocyte Medium, Defined
- CnT-02-3DP5, Epidermal Keratinocyte 3D Prime Medium, Defined, 500 ml
All media are designed for use in a 5% CO2 atmosphere.
Cultivation:
For cultivation instructions, please see also general cultivation protocol, on our resources section.
Passaging:
Recommended seeding density after passaging: 4 x 103 cells / cm2.
For passaging instructions, please see also general cultivation protocol, on our resources section.
Av. Time to Confluence:
6 to 8 days for monolayer culture of keratinocytes (depending on temperature, seeding density and protocol)
Longevity:
Keratinocytes guaranteed to provide 20 population doublings when grown in a PCT medium.
Storage / Shelf Life:
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Medium storage: basal medium (BM) at 4°C in the dark, supplements (S) at -20°C. For expiry date refer to medium label.
Supplemented medium: 4°C in the dark, use within 8 weeks of supplementation. Do not freeze.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Millicell-PCF inserts: room temperature. One year from date of purchase.
CnT-ST: room temperature. One year from date of purchase.
Thawing:
Recommended seeding density after thawing: 2 to 4 x 103 cells / cm2.
For thawing instructions, please see also general cultivation protocol, on our resourcses section.
Freezing:
Recommended freezing density: 1 x 106 cells / mL.
For freezing instructions for keratinocytes, please see also general cultivation protocol, on our resources section.
Quality Control:
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping Conditions:
Cells and supplements are shipped on dry ice.
Basal medium, Millicell-PCF inserts and stain solution are shipped at ambient temperature.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Thu, 2011/09/29
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If desired antibiotics / antimycotics can be added.
Related Product: 3D-HPEK-50: 3D Keratinocyte Kit including Human Primary Keratinocytes
Replacement Products:
Millipore: Millicell cell culture inserts PIHP01250, PCF inserts, 12 mm diameter, 0.4 µm pore size, pack of 50 inserts