3D-K-50
Printable Datasheet/Page
Product Title:
3D Keratinocyte Starter Kit without Human Primary Keratinocytes
3D keratinocyte starter Kit was developed for the establishment of 3 dimensional keratinocyte cultures on a porous membrane. Supplied with one 500 mL kit of CnT-57 medium (basal medium with frozen supplements), one 500 mL kit of CnT-02-3DP5 (basal medium with frozen supplements), stain solution and Millicell cell culture inserts.
Species:
Kit is developed for human keratinocytes but may be used for other species as well.
Pack Size:
3D-K-50 is a kit consisting of:
- 1 x CnT-57: 500 mL kit of CnT-57 PCT Epidermal Keratinocyte Medium, Low BPE
- 1 x CnT-02-3DP5: one 500 mL kit of CnT-02-3DP5 Epidermal Keratinocyte 3D Prime Medium, Defined
- 50 x Millipore Millicell-PCF inserts: standing cell culture inserts, polycarbonate membrane, 12 mm diameter, 0.4 µm pore size, sterile and individually blistered
- 1 x CnT-ST: stain solution with 3 mL stain solution I and 3 mL stain solution II
- 1 x 3D-K-50 Data sheet
- 1 x 3D-K-50 Protocol
Culture Medium:
Included medium in the kit:
CnT-57, PCT Epidermal Keratinocyte Medium, Low BPE
CnT-02-3DP5, Epidermal Keratinocyte 3D Prime Medium, Defined, 500 ml
Alternatively cells can also be cultivated in the media listed below:
- CnT-07, PCT Epidermal Keratinocyte Medium, Defined
- CnT-02-3DP1, Epidermal Keratinocyte 3D Prime Medium, Defined, 100 ml
All media are designed for use in a 5% CO2 atmosphere.
Cultivation:
For cultivation instructions, please see also general cultivation protocol, on our resources section.
Passaging:
Recommended seeding density after passaging: 4 x 103 cells / cm2.
For passaging instructions, please see also general cultivation protocol, on our resources section.
Storage / Shelf Life:
Medium storage: basal medium (BM) at 4°C in the dark, supplements (S) at -20°C. For expiry date refer to medium label.
Supplemented medium: 4°C in the dark, use within 8 weeks of supplementation. Do not freeze.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Millicell-PCF inserts: room temperature. One year from date of purchase.
CnT-ST: room temperature. One year from date of purchase.
Thawing:
Recommended seeding density after thawing: 2 to 4 x 103 cells / cm2.
For thawing instructions, please see also general cultivation protocol, on our resourcses section.
Freezing:
Recommended freezing density: 1 x 106 cells / mL.
For freezing instructions for keratinocytes, please see also general cultivation protocol, on our resources section.
Quality Control:
Free of bacteria, fungi and mycoplasma contamination.
Shipping Conditions:
Supplements are shipped on dry ice.
Basal medium, Millicell-PCF inserts and stain solution are shipped at ambient temperature.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Thu, 2011/09/29
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If desired antibiotics / antimycotics can be added.
Related Product:
Replacement Products:
Millipore: Millicell cell culture inserts PIHP01250, PCF inserts, 12 mm diameter, 0.4 µm pore size, pack of 50 inserts