Epidermal Keratinocyte 3D Prime Medium, Defined
CnT-02-3DP1 is a liquid culture medium kit including both basal medium and separate supplements. CnT-02-3DP1 is a fully defined, high calcium (1.2 mM) formulation. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds.
CnT-02-3DP1 is intended for use with CELLnTEC Primary Keratinocytes or your own keratinocytes isolated and grown in CELLnTEC PCT media (CnT-07 or CnT-57). Successful 3D growth requires a cell culture with a viable subset of undifferentiated progenitor cells. Keratinocytes isolated in non-CELLnTEC media may not contain enough of these cells to maintain the growth of a full thickness 3D model. Use of keratinocytes isolated and grown in other vendor’s medium may prove unsuccessful.
CnT-02-3DP1 is a 100 mL kit consisting of:
- CnT-BM.3-100: 100 mL CnT Basal Medium 3 (liquid)
- CnT-02-3DP1.S: containing three supplements (Supplement A, B and C; delivered frozen; to be added to 100 mL basal medium prior to use)
Developed for 3D (air / liquid interface) culture of epidermal keratinocytes on a porous membrane. Recommended membrane: Millipore Millicell Cell Culture Insert, PCF inserts, 0.4 μm. Cells should be established on the membrane in CnT-57 or CnT-07 medium, and then switched to CnT-02-3DP1 medium after reaching confluency. See our resources section for full protocol and training video for optimal performance.
Developed for 3D differentiation of human keratinocytes. May be used for other species as well (not yet tested).
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols and training video, please see our resources section.
CnT-BM.3-100 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.
CnT-02-3DP1.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag.
The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 8 weeks. Please do not freeze.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Tested by 3D cell cultivation in supplemented medium using CELLnTEC Kit and Protocols. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
CnT-BM.3-100 basal medium is shipped at ambient temperature.
CnT-02-3DP1.S supplements are shipped frozen.
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
For research use only. Not for use in therapy or diagnostics.