CELLnTEC has the following specialized epidermal keratinocyte media available for 2D and 3D keratinocyte culture.

Human Epidermal Keratinocytes (HPEKp) in CnT-57 after 20 population doublings (cells grown according to our recommended protocols and seeding densities)

Isolation & Proliferation

CnT-07; CnT-03 - Defined, PCT media. Where a completely defined environment and maximum keratinocyte purity is required. The PCT formulations of CnT-07 and CnT-03 provide excellent results through maximum retention of keratinocyte progenitors, and inhibition of fibroblasts.

CnT-57 - Low-BPE, PCT medium recommended where absolute best attachement, isolation efficiency, colony formation efficiency and longevity is required, the quality of the starting material is not optimal, and / or a low BPE concentration (5 to 8 x lower than competitors) is compatible with the experimental setup.

CnT-08; CnT-09 - Non-defined media optimized for the growth of sheep and dog keratinocytes.

Differentiation

CnT-02; CnT-33 - Defined media using a optimized basal formulation and supplements. These media do not contain PCT factors, and are intended for use when keratinocytes are induced to differentiate. 

CnT-02-3DP1; CnT-02-3DP5 - For 3D differentiation, please see our 3D Culture section.

As with all differentiation work, it is important that cells reach confluency before calcium levels are increased. Furthermore, and next to the addition of calcium, cells should also be switched to CnT-02, a non-PCT medium. For our recommended differentiation protocol, see our resources section.

Comparison of epdiermal keratinocytes isolated in 3 different culture media. CnT-07, a defined PCT medium, has established significantly more cells at the end of passage 1, (comparison provided by a customer).

Comparisons

For an independent evaluation (Löhberg et al 2009) that found CnT-57 and CnT-07 superior to other media tested for the isolation and culture of basal keratinocyte progenitors, click here.

For A comparison of colony formation in PCT and competing formulations, please click here.  For a comparison of growth in CnT-07 vs a leading competitor during the first 5 passages, click here.

Applications From The Literature

Stem cell isolation, transplantation in mouse: Strachan et al., 2010, Epidermal Cell Materials and Methods.  Identification of long-term regenerative cells (stem cells) in the skin, and transplantion with fibroblasts.

3D keratinocyte and melanocyte co-culture: Gunathilake et al., 2009, Journal of Investigative Dermatology.

Stem and transient amplifying cells from young and aged epidermis: Charruyer et al., 2009, Journal of Investigative Dermatology. Frequency and cycling of various progenitors in young vs aged skin.

Isolation of keratinocytes and generation of iPS cells: Li et al., 2009, Nature.

3D culture of mouse keratinocytes, wound healing: Kandyba et al., 2010, Methods in Molecular Biology.

To search our full list of publications for your own keywords, please visit our Publications Page.

Free Medium Samples

CELLnTEC is offering free samples to new customers who are interested to test the performance of our precision culture media.

When testing your free sample, there are a number of important considerations. Please see our resources section for Sample Testing Recommendations.

Order your Free Sample Today: Please contact your local distributor for a free sample. Please see our ordering section for the CELLnTEC product distributor in your area.