Epidermal Keratinocyte Medium, Defined
CnT-02 is a liquid culture medium kit including both basal medium and supplements. CnT-02 is a fully defined, low calcium (0.07 mM) formulation. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
CnT-02 is a 500 mL kit consisting of:
- CnT-BM.1: 500 mL CnT Basal Medium 1 (liquid)
- CnT-02.S: containing two supplements (Supplements A and B; delivered frozen; to be added to 500 mL basal medium prior to use)
Developed for differentiation of mouse and human keratinocytes. May also be used for mouse keratinocyte isolation.
Developed for mouse and human keratinocytes. May be used for other species as well (not yet tested).
This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.
CnT-BM.1 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.
CnT-02.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag.
The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 8 weeks. Please do not freeze.
Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Media functionality and cell morphology tested by cell cultivation over 2 passages in supplemented medium. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.
- CnT-BM.1 basal medium is shipped at ambient temperature.
- CnT-02.S supplements are shipped frozen.
For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.
For research use only. Not for use in therapy or diagnostics.