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CnT-07CF

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Product Title: 

PCT Epidermal Keratinocyte Medium, Defined, Calcium Free

CnT-07CF is a progenitor cell targeted liquid culture medium kit including both basal medium and supplements. CnT-07CF is a fully defined, calcium free formulation. It does not contain calcium or antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.

Pack Size: 

CnT-07CF is a 500 mL kit consisting of:

  • CnT-BM.4: 500 mL CnT Basal Medium 4, Calcium Free (liquid)

  • CnT-07.S: containing three supplements (Supplement A, B and C; delivered frozen; to be added to 500 mL basal medium prior to use) 

Product Use: 

Developed for maximum efficiency keratinocyte isolation and growth. Requires the addition of calcium for cell growth – recommended concentration 0.07 mM. For 2D differentiation experiments we recommend to use CnT-02 or CnT-02CF (calcium free) Epidermal Keratinocyte Medium.

Tissue Type: 
Epidermal
Media Type: 
PCT
Defined: 
Yes
Species: 

Developed for human and mouse keratinocytes. May be used for other species as well (not yet tested).

Culture Conditions: 

This medium is designed for use in a 5% CO2 atmosphere. For suggested cell culture protocols, please see our resources section.

Storage / Shelf Life: 

  • CnT-BM.4 basal medium: Store at 4°C in the dark. For expiry date, please see label on bottle. Please do not freeze.

  • CnT-07.S supplements: Store at -20°C. For expiry date, please see label on tube and in bag. 

  • The complete medium (basal medium supplemented with the corresponding supplements) must be stored at 4°C in the dark. Storage in this manner will provide a minimum shelf life of 8 weeks. Please do not freeze. 

Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.

Quality Control: 

Media functionality and cell morphology tested by cell cultivation over 2 passages in supplemented medium. Osmolality, pH and concentration of various ions are tested. Free of bacteria, fungi and mycoplasma contamination.

Shipping Conditions: 

  • CnT-BM.4 basal medium is shipped at ambient temperature.

  • CnT-07.S supplements are shipped frozen.

Note: 

For routine cell cultivation, CELLnTEC recommends to work without antibiotics / antimycotics.

Intended Use: 

For research use only. Not for use in therapy or diagnostics.

Last Update: 
Thu, 2011/09/29
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