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MPEK-BL6

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Product Title: 

Epidermal Keratinocyte Progenitors

Mouse epidermal keratinocyte progenitor cells, cryo preserved at passage 25. Long-Term cells supplied with one 500 mL kit of medium (basal medium with frozen supplements).

Species: 

Mouse, C57BL/6, embryonic day 18.5

Pack Size: 

MPEK-BL6 is a kit consisting of:

  • 1 x MPEK-BL6.C: > 5 x 105 viable cells per vial (1mL)
  • 1 x CnT-07: 500 mL kit of CnT-07 medium
Culture Medium: 

Included medium in the kit:

CnT-07, PCT Epidermal Keratinocyte Medium, Defined

 

Alternatively cells can also be cultivated in the medium listed below:

  • CnT-57, PCT Epidermal Keratinocyte Medium, Low BPE
  • CnT-57CF, PCT Epidermal Keratinocyte Medium, Low BPE, Calcium Free
  • CnT-07CF, PCT Epidermal Keratinocyte Medium, Defined, Calcium Free
  • CnT-02, Epidermal Keratinocyte Medium, Defined
  • CnT-02CF, Epidermal Keratinocyte Medium, Defined, Calcium Free
  • CnT-02-3DP1, Epidermal Keratinocyte 3D Prime Medium, Defined, 100 ml
  • CnT-02-3DP5, Epidermal Keratinocyte 3D Prime Medium, Defined, 500 ml
Cultivation: 

For cultivation instructions, please see general cultivation protocol, on our recources section.

Passaging: 

Recommended seeding density after passaging: 4 to 6 x 103 cells / cm2.

For passaging instructions, please see general cultivation protocol, on our resources section.

Av. Time to Confluence: 
5 to 7 days (depending on temperature, seeding density and protocol)
Longevity: 

Guaranteed to provide an additional 6 months of continuous growth when used with the recommended medium.

Storage / Shelf Life: 

Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.

Medium storage: refer to medium label and data sheet.

Thawing: 

Recommended seeding density after thawing: 8 x 103 cells / cm2.

For thawing instructions, please see general cultivation protocol, on our resources section.

Freezing: 

Recommended feezing density: 1 x 106 cells / mL.

For freezing instructions, please see general cultivation protocol, on our resources section.

Quality Control: 

Free of bacteria, fungi and mycoplasma contamination.

Shipping Conditions: 

Cells and supplements are shipped on dry ice.

Basal medium is shipped at ambient temperature.

Comments: 

These keratinocytes have been shown to express keratin, and establish adhesion molecules including catenins, cadherins and desmoglein when induced to differentiate.

For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.

Starting in December 2010: medium change in the kit. Now including CnT-07 medium. If CnT-02 is required, kit can be purchased with CnT-02 upon request.

Intended Use: 

For research use only. Not for use in therapy or diagnostics.

Last Update: 
Mon, 2010/12/06
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