Epidermal Keratinocyte Progenitors
Rat epidermal keratinocyte progenitor cells, cryo preserved at passage 25. Long-Term cells supplied with one 500 mL kit of medium (basal medium with frozen supplements).
RPEK-WIS is a kit consisting of:
- 1 x RPEK-WIS.C: > 5 x 105 viable cells per vial (1mL)
- 1 x CnT-03: 500 mL kit of CnT-03 medium
Included medium in the kit:
CnT-03, PCT Epidermal Keratinocyte Medium, Defined
Alternatively cells can also be cultivated in the media listed below:
CnT-33, Epidermal Keratinocyte Medium, Defined
For cultivation instructions, please see general cultivation protocol, on our recources section.
Recommended seeding density after passaging: 4 to 6 x 103 cells / cm2.
For passaging instructions, please see general cultivation protocol, on our resources section.
Guaranteed to provide an additional 6 months of continuous growth when used with the recommended medium.
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Medium storage: refer to medium label and data sheet.
Recommended seeding density after thawing: 8 x 103 cells / cm2.
For thawing instructions, please see general cultivation protocol, on our resources section.
Recommended feezing density: 1 x 106 cells / mL.
For freezing instructions, please see general cultivation protocol, on our resources section.
Free of bacteria, fungi and mycoplasma contamination.
Cells and supplements are shipped on dry ice.
Basal medium is shipped at ambient temperature.
For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.
For research use only. Not for use in therapy or diagnostics.