HPEKas.15
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Product Title:
Epidermal Keratinocyte Progenitors, Adult, Single Donor
Normal human epidermal keratinocyte progenitor cells, isolated from adult donor material, cryo preserved at P2. Primary cells supplied with one 500 mL kit of medium (basal medium with frozen supplements).
Warning:
This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species:
Human, adult, single donor, abdominal skin
Pack Size:
HPEKas.15 is a kit consisting of:
- 3 x HPEKas.05.C: 3 vials > 5 x 105 viable cells per vial (1 mL)
- 1 x CnT-57: 500 mL kit of CnT-57 medium
Culture Medium:
Included medium in the kit:
CnT-57, PCT Epidermal Keratinocyte Medium, Low BPE
Alternatively cells can also be cultivated in the media listed below:
- CnT-57CF, PCT Epidermal Keratinocyte Medium, Low BPE, Calcium Free
- CnT-07, PCT Epidermal Keratinocyte Medium, Defined
- CnT-07CF, PCT Epidermal Keratinocyte Medium, Defined, Calcium Free
- CnT-02, Epidermal Keratinocyte Medium, Defined
- CnT-02CF, Epidermal Keratinocyte Medium, Defined, Calcium Free
- CnT-02-3DP1, Epidermal Keratinocyte 3D Prime Medium, Defined, 100 ml
- CnT-02-3DP5, Epidermal Keratinocyte 3D Prime Medium, Defined, 500 ml
Cultivation:
For cultivation instructions, please see general cultivation protocol, on our resources section.
Passaging:
Recommended seeding density after passaging: 4 x 103 cells / cm2.
For passaging instructions, please see general cultivation protocol, on our resources section.
Av. Time to Confluence:
6 to 8 days (depending on temperature, seeding density and protocol)
Longevity:
Guaranteed to provide 20 population doublings when grown in a PCT medium.
Storage / Shelf Life:
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Medium storage: refer to medium label and data sheet.
Thawing:
Recommended seeding density after thawing: 4 x 103 cells / cm2.
For thawing instructions, please see general cultivation protocol, on our resources section.
Freezing:
Recommended freezing density: 1 x 106 cells / mL.
For freezing instructions, please see general cultivation protocol, on our resources section.
Quality Control:
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping Conditions:
Cells and supplements are shipped on dry ice.
Basal medium is shipped at ambient temperature.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Fri, 2010/10/08
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For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.