HDFs.15
Printable Datasheet/Page
Product Title:
Dermal Fibroblasts, Single Donor
Normal human dermal fibroblasts, cryo preserved at P2. Primary cells supplied with one 500 mL kit of medium (basal medium with frozen supplements).
Warning:
This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species:
Human, single donor, foreskin
Pack Size:
HDFs.15 is a kit consisting of:
- 3 x HDFs.05.C: 3 vials > 5 x 105 viable cells per vial (1 mL)
- 1 x CnT-05: 500 mL kit of CnT-05 medium
Culture Medium:
Included medium in the kit:
CnT-05, Dermal Fibroblast Medium, Non-defined (contains serum)
Cultivation:
For cultivation instructions, please see general cultivation protocol, on our resources section.
Passaging:
Recommended seeding density after passaging: 4 x 103 cells / cm2.
For passaging instructions, please see general cultivation protocol, on our resources section.
Av. Time to Confluence:
6 to 8 days (depending on temperature, seeding density and protocol)
Longevity:
Guaranteed to provide 20 population doublings when grown in the recommended medium.
Storage / Shelf Life:
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Medium storage: refer to medium label and data sheet.
Thawing:
Recommended seeding density after thawing: 4 x 103 cells / cm2.
For thawing instructions, please see general cultivation protocol, on our resources section.
Freezing:
Recommended freezing density: 1 x 106 cells / mL.
For freezing instructions, please see general cultivation protocol, on our resources section.
Quality Control:
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping Conditions:
Cells and supplements are shipped on dry ice.
Basal medium is shipped at ambient temperature.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Mon, 2010/06/07
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For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics. For isolation, However, we recommend the use of antibiotics / antimycotics up to passage 2.