General Cell Culture Reagents
For your cell culture work, CELLnTEC offers a variety of reagents for your needs. Click on the catalog numbers of the corresponding products below to obtain more information.
|CnT-ABM10||Pen / Strep / Amphotericin B Solution, Single Aliquots||
10 x 2.5 mL of frozen concentrate (200 x).
|CnT-Accutase-100||Cell Detachment Solution||
100 mL ready-to-use Accutase solution, frozen
|CnT-BPE-5||Bovine Pituitary Extract (BPE)||
5 x 1 mL of frozen BPE
|CnT-CRYO-50||CRYO-Defined, Animal Component Free Freezing Medium||
50 mL liquid freezing medium
|CnT-DNP-10||Dispase II, Neutral Protease, 20 x||
10 mL solution, 20 x
|CnT-GAB10||Gentamycin / Amphotericin B Solution, Single Aliquots||
10 x 1 mL of frozen concentrate (500 x).
|CnT-ST-100||Stain Kit, including Solution I and Solution II||
100 mL staining kit
Antibiotics / Antimycotics
It is now widely known that traditional penicillin / streptomycin mixtures are not well suited to the heat and pH of cell culture (they rapidly lose activity, and encourage bacterial resistance). Gentamycin is a favored cell culture antibiotic, due to its better thermo stability, acidic resistance, broader effective spectrum, and longer shelf life at 4°C in the dark. For further information about the advantages of Gentamycin, click here.
For CELLnTEC's recommendations on the use of antibiotics and antimycotics in routine cell culture, see the relevant point in the FAQ's.
Cryopreservation / Freezing Medium
Developed using only synthetic compounds, the xeno-free CRYO-Defined (Data Sheet: CnT-CRYO-50) is an advanced new freezing medium that is fully defined, and thus no longer depends on the use of variable additives such as serum. In testing this medium has been found to deliver equal or better cell recovery after thawing than traditional high-serum freezing media. Click here for more information.
Dispase II (Neutral Protease) is an optimal emzyme for isolation of primary cells. it provides gentle but effective seperation of epithelial and stromal cells.
Our dispase product CnT-DNP-10 is a 10 mL, 20 x solution, that is tested for activity in cell culture isolation. It is provided at an optimal concentration for use in primary epithelial cell isolation.
CnT-DNP-10 is sufficient for up to 20 isolations. For complete technical information, please see the datasheet.
Special offer: 15% off all dispase ordered up until 18th November 2011!
Accutase Cell Detachement Solution
Accutase® has been developed to meet the most demanding needs for gentle and effective detachment of adherent cells and can be used as a direct replacement for trypsin solution (Data Sheet: CnT-Accutase-100). It is useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and has been proven effective in detaching: fibroblasts, epithelial cells and a whole range of other cell types. Find more information here.
Accutase is a registered trademark of Innovative Cell Technologies.
Bovine Pituatry Extract (BPE)
BPE is a well known non-defined cell culture supplement, which contains growth factors and has a beneficial effect on attachment and growth of primary cells and cell lines (Data Sheet: CnT-BPE-5). CELLnTEC's media do not require any additional supplements. However, in other situations where an optimized medium is not available, the cells of a specific species are difficult to culture or the quality of the starting tissue sample for isolation is not optimal, you may wish to evaluate an additional BPE supplementation.
Stain Solution Kit
The stain kit can be used to stain fixed cells.It consists of two different ready-to-use components which can be re-used up to five times (Data Sheet: CnT-ST-100). The stain kit can be used for cells growing on different surfaces:
• Cell culture inserts. The stain solutions can be used on polycarbonate and PET membranes. The cells can be
stained on mixed cellulose ester membranes, only when ethanol is utilizes as a fixing agent.
• Cells growing on cell culture plastic surface can be stained to monitor for colony forming efficiency, growth pattern etc.
• Cells growing on chamber slides.
For staining procedures, please see the general staining protocol on our resources section.