CnT-Accutase-100
Printable Datasheet/Page
Product Title:
Cell Detachment Solution
Accutase® is a ready-to-use cell detachment solution, that contains proteoloytic and collagenolytic enzymes. Due to the invertebrate origin (crabs) it is free of mammalian and bacterial derived proteins. Accutase can serve as a direct replacement for trypsin.
Accutase is a Registered Trade Mark of Innovative Cell Technologies Inc.
Product Use:
It can be used for the detachment of cells from all types of cell culture plastics, including adhesion coated plastic ware. Accutase is a mild detachment enzyme that is very useful for the detachment of cells with following cell surface marker analysis. It is also useful for the separation of cell clumps for further analysis like cell counting with and without viability stain or flow cytometric analysis.
For passaging protocol, please see general cultivation protocol, in our resources section.
Pack Size:
100 mL ready-to-use Accutase solution, frozen
Activity / Concentration:
500 to 720 U / mL / 1 x Accutase
Storage / Shelf Life:
Store at -20°C. See label for expiry date.
Once thawed Accutase can be stored two month at 4°C. Avoid repeated freeze and thaw cycles.
Thaw at 4°C, do not heat warmer than room temperature.
Quality Control:
Tested for sterility, pH 6.8 to 7.8.
Shipping Conditions:
CnT-Accutase-100 is shipped frozen.
Intended Use:
For research use only. Not for use in therapy or diagnostics.
Last Update:
Thu, 2010/12/02
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Solution in DPBS without Ca2+ and Mg2+, with 0.5 mM EDTA and 3 mg / L Phenol Red