Isolation and Proliferation
Primary human urothelial cells (HBEP), passage 2, CnT-58 culture medium
|Human keratinocytes from foreskin isolated and cultivated in the indicated media. The defined CnT-07 and the Low-BPE CnT-57 showing elongated longevity and higher cumulative population doublings when compared to High-BPE formulations KGM (Cambrex, Lonza) or KSFM (Invitrogen).|
For our recommended isolation and cultivation protocols, please visit our resources section.
CELLnTEC media are designed to mimic specific in vivo micro-environments as closely as possible. Our culture media optimized for isolation and proliferation of primary cells are known as Progenitor Cell Tartgeted (PCT) media. These media are specifically designed to select progenitor cells, and to effectively maintain them in an proliferative state using several mechanisms known to be active in the stem cell niche.
Progenitor cell targeted media for isolation and proliferation are available in either fully defined, or low BPE formulations. Each version has specific capacities, which influence the decision of which to choose for a specific situation (see also the Media Selection Guide):
- PCT Media (Defined): used for isolation and proliferation of primary cells, these media are fully defined, offering complete knowledge and control of the environment used during experimentation. They are highly selective for epithelial cells, and do not allow significant growth of stromal cell types such as fibroblasts.
- PCT Media (Low-BPE): also used for isolation and proliferation of primary cells, these media contain a low concentration of Bovine Pituitary Extract. Unlike traditional media, the addition of BPE is not intended primarily as a source of growth factors, and as such only a low concentration (typically 5 to 8 x lower than traditional media) is used. The addition of BPE aids attachment and migration, and results in improved colony forming efficiency and maximum cell yield.
Due to the otherwise identical formulations of the defined and low-BPE media, cells can be changed easily from one to the other. For example in certain labs cells are routinely isolated and maintained in a low-BPE medium to maximize isolation efficiency and colony formation, and then switched to the fully defined medium after 2 passages to allow full control of the experimental system.