Cultivation Protocols

Recommended Seeding Densities (PDF)

General Cultivation Protocol for Primary and Long-Term Cells (PDF)

Special Cultivation Protocol for Bladder Epithelial Cells (PDF)

Isolation Protocols

Recommended Seeding Densities (PDF)

Epidermal Keratinocyte Isolation, Human (PDF)

Epidermal Keratinocyte Isolation, Mouse (PDF)

Epidermal Keratinocyte Isolation, Dog (PDF)

Airway (Small) Epithelium Cell Isolation, Human (PDF)

Airway (Large) Epithelium Cell Isolation, Human (PDF)

Bladder Epithelium Cell Isolation, Human (PDF)

Bladder Epithelium Cell Isolation, Rat (PDF)

Corneal Epithelium Cell Isolation (PDF)

Fibroblast Isolation, Human (PDF)

Mammary Epithelium Cell Isolation (PDF)

Oral Epithelium Cell Isolation (PDF)

Prostate Epithelium Cell Isolation (PDF)

2D Differentiation Protocols

2D Differentiation of Keratinocytes in CnT-07/CnT-57 (PDF)

2D Differentiation of Other Epithelial Cells grown in a PCT medium (PDF)

3D Differentiation Protocols

For the 3D Epidermal Cell Culture Training Video, please click here.

3D Epidermal Cell Cultures: Starter Kit with HPEK Keratinocytes (PDF)

3D Epidermal Cell Cultures: Starter Kit without keratinocytes (PDF)

3D Large Airway Cultures, Rat (PDF)

Routine Histology and Staining of 3D Cell Cultures (PDF)

Other Useful Protocols

OCT Embedding of 3D Cultures for Cryo-Sectioning (PDF)

Lipid Extraction (PDF)

Keratinocyte Transfection Using PEI (PDF)

CELLnTEC Media Composition

For more info to CELLnTEC's Media Composition, click here.