In vitro approaches (to vitiligo)
Institution:
Inserm U876, Centre de référence des maladies rares de la peau, Université V Segalen Bordeaux
Country:
France
Year:
2010
Journal Name:
Vitiligo (Book, Chapter 2.2.5)
The complexity of cell culture models varies from monolayers for adherent cells or suspensions for nonadherent cells, to cocultures or to epidermal reconstructs (3D model).
Cocultures and epidermal reconstructs allow the srudy of paracrine or contact cell-cell effects.
The tests on nonepidermal nonadherent cells, such as peripheral blood mononuclear cells (PBMC) expand the view, going beyond melanogenesis-associated metabolisms.
The in vitro studies on hair follicle melanocytes help understand the maturation and differentiation of melanocytes and to unveil the defective steps of growth and migration in melanocyte precursors during the repigmentation.
New analytic techniques are emerging which can be applied for investigating vitiligo.
Tissue Type:
Epidermal
Tissue Info:
Cells received from lesional or nonlesional skin of generalized vitiligo patients
Species:
Human
CELLnTEC Products:
Product Use:
Isolation and cultivation for consequent reconstruction of epidermis or to start cocultures for functional studies (see protocol in the corresponding book chapter).
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