Recovery of primary human keratinocytes 6 days post thawing. A: Frozen in traditional 20% freezing medium. B: Frozen in CRYO-Defined xeno-free freezing medium.

Developed using only synthetic compounds, the xeno-free CRYO-Defined freezing medium is an advanced new formulation that is fully defined, and thus no longer depends on the use of variable additives such as serum.

In testing this medium has been found to deliver equal or better cell recovery after thawing than traditional high-serum freezing media (see image).

Printable datasheet

CRYO-Defined, Animal Component Free Freezing Medium

CRYO-Defined is a 2 x concentrate, cryo preservation medium containing 20% DMSO and all the necessary components to protect cells during freezing and thawing. This freezing medium is chemically defined and animal component free. It is ready to use as provided.

Tissue type
Freezing Media
Pack size
50 mL
Storage / Shelf life
Store at -20°C in the dark. See labels for expiry date. Once thawed, use within 6 months.
Quality control
Growth and morphology of cells tested after thawing using the recommended protocol. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Shipped frozen.
Intended use
For research use only. Not for use in therapy or diagnostics.
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