HIGHLIGHTS:
- Chemically defined, contains no components of animal or human origin
- Specifically designed for balanced co-culture of keratinocytes and fibroblasts
- Fully supplemented, one bottle ready-to-use
CnT-Prime Epithelial/Stromal Co-Culture Medium
Co-culture of epithelial and stromal cells in 2D culture.
| Catalog | CnT-PR-CC |
|---|---|
| Content | 500 ml, Frozen medium |
Description
CnT-PR-CC is a fully defined medium for the co-culture of primary human keratinocytes and fibroblasts.
Specifications
- Tissue type
- Fibroblasts & Keratinocytes
- Application
- Co-culture
- Serum
- No
- BPE
- No
- Free of Human & Animal Component
- Yes
- Chemically defined
- Yes
- Clinically upgradable
- Yes
- Volume
- 500 ml
- Component(s)
- Frozen Bottle & Ready-to-use
- Quality level
- Research Grade
Scientific resources
CnT-PR-CC delivers balanced co-culture of keratinocytes & fibroblasts.
CnT-PR-CC is specifically formulated to allow balanced co-culture of both epithelial and stromal cells in a fully defined environment.Primary keratinocytes and fibroblasts are initially expanded in their respective media. When seeded at a 50:50 ratio into standard 2D culture, both cell types proliferate.
At confluency, the cultures display the characteristic keratinocyte progenitor cell colonies (small and tight cobblestone morphology), surounded by fibroblasts (see image).
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details.
Like to try a test sample? Click here for more information.
Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Scientific Literature
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion | A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion | 2018 | Epidermal |
| An improved multicellular human organoid model for the study of chemical effects on palatal fusion | Cynthia J. Wolf, Hunter Fitzpatrick, Carrie Becker, Jessica Smith, Carmen Wood | 2023 | Epidermal |
| Development of an organotypic stem cell model for the study of human embryonic palatal fusion | Cynthia J. Wolf, David G. Belair, Carrie M. Becker, Kaberi P. Das, Judith E. Schmid, Barbara D. Abbott | 2018 | Epidermal |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |