Provides all the components required for establishing balanced co-culture of primary keratinocytes and melanocytes in a fully defined environment.
| Catalog | PR2D-KM |
|---|---|
| Content | HPEKp & HEMas cells, CnT-PR, CnT-40 & CnT-PR-KM media |
The Keratinocyte/Melanocyte 2D Co-culture Starter Kit (PR2D-KM) provides all the components required for establishing balanced co-culture of primary keratinocytes and melanocytes in a fully defined environment. The kit includes primary human keratinocyte and melanocyte cells, CnT-PR and CnT-40 media for monolayer culture and CnT-PR-KM co-culture medium. The kit is designed to enable you to establish 2D co-culture models in your lab with ease and fidelity. Note: The cells are of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Balanced co-culture of keratinocytes & melanocytes obtained by PR2D-KM starter kit
The Keratinocyte/Melanocyte 2D Co-culture Starter Kit PR2D-KM enables you to establish 2D co-culture models in your lab with ease and fidelity.
The primary keratinocytes and melanocytes are initially expanded in their respective proliferation media following our recommended general protocol.
These primary cells proliferate together when seeded at an equivalent ratio into standard 2D culture with CnT-PR-KM co-culture medium.
The standard keratinocyte media contain ingredients that inhibit melanocyte growth or drive immediate differentiation. Consequently, the melanocytes fail to survive in 2D culture with keratinocytes. The CnT-PR-KM co-culture medium in the kit is tailored to maintain a balanced co-culture of primary human keratinocytes and melanocytes for up to a week, in a fully defined environment.
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |
