Contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.
| Catalog | PR3D-K-50 |
|---|---|
| Content | CnT-PR & CnT-PR-3D media, 48 x Cell culture inserts |
3D keratinocyte starter Kit was developed for the establishment of 3-dimensional keratinocyte cultures with improved barrier function. Kit includes CnT-Prime medium (CnT-PR) for monolayer culture, CnT-Prime 3D airlift medium (CnT-PR-3D), and 48 cell culture inserts (0.4 um pore size). Kit does not contain primary keratinocytes. Optional extra: CnT-ST-100 stain solution to evaluate confluency prior to airlift.
Improved barrier as seen by raman spectroscopy penetration profiles.
The 3D Starter kit without primary keratinocytes (PR3D-K-50) contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.
Early passage human keratinocytes growing in CnT-PR medium are also required.
The kit contains CnT-PR proliferation medium, CnT-PR-3D barrier medium, and 48 inserts for air-lift culture. For details, please see the datasheet.
The 3D models can be routinely established using our recommended protocol.
Following this protocol, the 3D models deliver up to a 50% better barrier function than traditional media. More detailed raman spectroscopy results can be found in the following link:
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |